Can lentivirus infect non-dividing cells?

Lentiviruses share the common characteristic of infecting non-dividing target cells, distinguishing them from the oncogenic retroviruses which only productively infect dividing cells. The search for determinants for infection of non-dividing cells has produced a number of candidates.

Do retroviruses infect non-dividing cells?

The dependence of retroviral replication on cell proliferation was described as early as 1958, although different classes of retroviruses are able to infect non-dividing cells with different efficiencies.

How do lentiviruses enter cells?

More specifically, lentiviruses attach to the CD4 glycoproteins on the surface of a host’s target cell. The viral material is then injected into the host cell’s cytoplasm. Within the cytoplasm, the viral reverse transcriptase enzyme performs reverse transcription of the viral RNA genome to create a viral DNA genome.

Is lentiviral transduction permanent?

Lentiviruses—a subclass of retroviruses—have the ability to permanently integrate into the genome of the host cell.

Can adenovirus infect non dividing cells?

Adenoviruses and Adeno-Associated Viruses (AAVs) are two types of viral vectors used for gene delivery. Both of these recombinant viral systems have the ability to infect a broad range of hosts, including dividing and non-dividing cells, without integrating with the host genome.

Why do retroviruses only infect dividing cells?

The main reason why MMLV viruses can only infect non-dividing cells is that they are unable to cross the nuclear membrane and can only achieve completion of the infection with provirus integration during cell division. Retroviruses enter the cells via cell fusion of the envelope protein with the cell membrane.

How does lentivirus integrate into genome?

Where does lentivirus integrate? Genome-wide studies of viral integration have shown that lentiviruses most often integrate into actively transcribed genes, and that this preference is conserved across target species.

How long does lentiviral transduction last?

For LV transduction, the longer you leave the virus in contact with the cells, the better your transduction will be. Christopher gave a good guide above, normally I would see some expression at 24h and maximum expression at 48-72 hours.

Does lentivirus trigger immune response?

Lentiviral vectors can trigger an adaptive immune response, which can be characterized as either humoral or cell-mediated.